191615

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1099465..1100048 Replicon chromosome
Accession NZ_CP069692
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JSU10_RS05255 Protein ID WP_000254745.1
Coordinates 1099713..1100048 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag JSU10_RS05250 Protein ID WP_000581939.1
Coordinates 1099465..1099713 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS05240 (1095804) 1095804..1097105 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JSU10_RS05245 (1097153) 1097153..1099387 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JSU10_RS05250 (1099465) 1099465..1099713 + 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JSU10_RS05255 (1099713) 1099713..1100048 + 336 WP_000254745.1 endoribonuclease MazF Toxin
JSU10_RS05260 (1100119) 1100119..1100910 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JSU10_RS05265 (1101138) 1101138..1102775 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JSU10_RS05270 (1102863) 1102863..1104161 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T191615 WP_000254745.1 NZ_CP069692:1099713-1100048 [Escherichia coli H20]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T191615 NZ_CP091839:c2238543-2238247 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT191615 WP_000581939.1 NZ_CP069692:1099465-1099713 [Escherichia coli H20]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT191615 NZ_CP091839:c2238245-2237850 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References