Detailed information of TA system TA191610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 9130..9595
• Replicon: plasmid pMUB-MIN10-MCR
• Accession: NZ_CP069680
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: JSU12_RS26320
• Protein ID: WP_001230707.1
• Coordinates: 9344..9595 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: JSU12_RS26315
• Protein ID: WP_000801440.1
• Coordinates: 9130..9357 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS26275 (JSU12_26330)	4360..4575	-	216	WP_000760375.1	EexN family lipoprotein	-
JSU12_RS26280 (JSU12_26335)	4572..4865	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
JSU12_RS26285 (JSU12_26340)	4942..6171	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
JSU12_RS26290 (JSU12_26345)	6175..6678	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
JSU12_RS26295 (JSU12_26350)	7039..7386	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
JSU12_RS26300 (JSU12_26355)	7383..7631	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
JSU12_RS26305 (JSU12_26360)	7936..8286	-	351	WP_000854259.1	hypothetical protein	-
JSU12_RS26310 (JSU12_26365)	8377..8676	-	300	WP_000650309.1	hypothetical protein	-
JSU12_RS26315 (JSU12_26370)	9130..9357	-	228	WP_000801440.1	hypothetical protein	Antitoxin
JSU12_RS26320 (JSU12_26375)	9344..9595	-	252	WP_001230707.1	hypothetical protein	Toxin
JSU12_RS26325 (JSU12_26380)	9592..10135	-	544	Protein_14	hypothetical protein	-
JSU12_RS26330 (JSU12_26385)	10240..10755	-	516	WP_001025395.1	J domain-containing protein	-
JSU12_RS26335 (JSU12_26390)	12170..13036	+	867	WP_001353743.1	replication initiation protein	-
JSU12_RS26340 (JSU12_26395)	13049..13453	+	405	WP_012421203.1	hypothetical protein	-
JSU12_RS26345 (JSU12_26400)	13588..14115	+	528	WP_208207915.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33305	33305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T191610 WP_001230707.1 NZ_CP069680:c9595-9344 [Escherichia coli O126:H45]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T191610 NZ_CP091839:582504-582680 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT191610 WP_000801440.1 NZ_CP069680:c9357-9130 [Escherichia coli O126:H45]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT191610 NZ_CP091839:582726-583142 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9