Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 7039..7631 Replicon plasmid pMUB-MIN10-MCR
Accession NZ_CP069680
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag JSU12_RS26300 Protein ID WP_000520549.1
Coordinates 7383..7631 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag JSU12_RS26295 Protein ID WP_000681613.1
Coordinates 7039..7386 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS26265 (JSU12_26320) 3045..3371 - 327 WP_000008647.1 TrbC/VirB2 family protein -
JSU12_RS26270 (JSU12_26325) 3432..4004 - 573 WP_227537772.1 lytic transglycosylase domain-containing protein -
JSU12_RS26275 (JSU12_26330) 4360..4575 - 216 WP_000760375.1 EexN family lipoprotein -
JSU12_RS26280 (JSU12_26335) 4572..4865 - 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
JSU12_RS26285 (JSU12_26340) 4942..6171 - 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
JSU12_RS26290 (JSU12_26345) 6175..6678 - 504 WP_000781813.1 relaxosome-coupling protein TaxA -
JSU12_RS26295 (JSU12_26350) 7039..7386 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JSU12_RS26300 (JSU12_26355) 7383..7631 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
JSU12_RS26305 (JSU12_26360) 7936..8286 - 351 WP_000854259.1 hypothetical protein -
JSU12_RS26310 (JSU12_26365) 8377..8676 - 300 WP_000650309.1 hypothetical protein -
JSU12_RS26315 (JSU12_26370) 9130..9357 - 228 WP_000801440.1 hypothetical protein -
JSU12_RS26320 (JSU12_26375) 9344..9595 - 252 WP_001230707.1 hypothetical protein -
JSU12_RS26325 (JSU12_26380) 9592..10135 - 544 Protein_14 hypothetical protein -
JSU12_RS26330 (JSU12_26385) 10240..10755 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33305 33305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T191609 WP_000520549.1 NZ_CP069680:c7631-7383 [Escherichia coli O126:H45]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T191609 NZ_CP091839:c370514-370407 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT191609 WP_000681613.1 NZ_CP069680:c7386-7039 [Escherichia coli O126:H45]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT191609 NZ_CP091839:370561-370627 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References