Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 14274..14875 Replicon plasmid pMUB-MIN10-2
Accession NZ_CP069679
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag JSU12_RS25810 Protein ID WP_001216045.1
Coordinates 14495..14875 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JSU12_RS25805 Protein ID WP_001190712.1
Coordinates 14274..14495 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS25760 (JSU12_25815) 9366..9626 + 261 WP_089540083.1 eaa protein -
JSU12_RS25765 (JSU12_25820) 9626..9907 + 282 WP_000002126.1 ASCH domain-containing protein -
JSU12_RS25770 (JSU12_25825) 9964..10224 + 261 WP_001369498.1 hypothetical protein -
JSU12_RS25775 (JSU12_25830) 10231..10605 + 375 WP_000988652.1 hypothetical protein -
JSU12_RS25780 (JSU12_25835) 10587..11261 + 675 WP_032236592.1 hypothetical protein -
JSU12_RS25785 (JSU12_25840) 11258..11620 + 363 WP_001261543.1 hypothetical protein -
JSU12_RS25790 (JSU12_25845) 12696..13052 - 357 WP_001062545.1 hypothetical protein -
JSU12_RS25795 (JSU12_25850) 13053..13637 - 585 WP_023356284.1 hypothetical protein -
JSU12_RS25800 (JSU12_25855) 13812..14201 + 390 WP_000506730.1 S24 family peptidase -
JSU12_RS25805 (JSU12_25860) 14274..14495 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JSU12_RS25810 (JSU12_25865) 14495..14875 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JSU12_RS25815 (JSU12_25870) 14880..15059 + 180 WP_000113018.1 hypothetical protein -
JSU12_RS25820 (JSU12_25875) 15087..16130 + 1044 WP_208207911.1 DUF968 domain-containing protein -
JSU12_RS25825 (JSU12_25880) 16219..16671 + 453 WP_032165150.1 Late promoter-activating protein -
JSU12_RS25830 (JSU12_25885) 16708..17883 - 1176 WP_089581153.1 RNA-guided endonuclease TnpB family protein -
JSU12_RS25835 (JSU12_25890) 18090..19283 + 1194 WP_000219622.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..98084 98084
- flank IS/Tn - - 16708..17883 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T191608 WP_001216045.1 NZ_CP069679:14495-14875 [Escherichia coli O126:H45]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T191608 NZ_CP091839:c369979-369872 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT191608 WP_001190712.1 NZ_CP069679:14274-14495 [Escherichia coli O126:H45]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT191608 NZ_CP091839:370026-370092 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References