Detailed information of TA system TA191604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 90689..91314
• Replicon: plasmid pMUB-MIN10-1
• Accession: NZ_CP069678
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JSU12_RS25350
• Protein ID: WP_000911313.1
• Coordinates: 90916..91314 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JSU12_RS25345
• Protein ID: WP_000450520.1
• Coordinates: 90689..90916 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS25345 (90689)	90689..90916	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JSU12_RS25350 (90916)	90916..91314	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JSU12_RS25355 (91323)	91323..93476	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
JSU12_RS25360 (93729)	93729..94460	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
JSU12_RS25365 (94492)	94492..94989	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul3 / ant(3'')-Ia / dfrA1 / tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..146908	146908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T191604 WP_000911313.1 NZ_CP069678:90916-91314 [Escherichia coli O126:H45]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T191604 NZ_CP091836:c4125634-4125416 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT191604 WP_000450520.1 NZ_CP069678:90689-90916 [Escherichia coli O126:H45]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT191604 NZ_CP091836:c4126034-4125660 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2