Detailed information of TA system TA191594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3809330..3810167
• Replicon: chromosome
• Accession: NZ_CP069677
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JSU12_RS18625
• Protein ID: WP_000227784.1
• Coordinates: 3809625..3810167 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JSU12_RS18620
• Protein ID: WP_001297137.1
• Coordinates: 3809330..3809641 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS18595 (3804350)	3804350..3805297	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
JSU12_RS18600 (3805319)	3805319..3807310	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JSU12_RS18605 (3807300)	3807300..3807914	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JSU12_RS18610 (3807914)	3807914..3808243	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JSU12_RS18615 (3808255)	3808255..3809145	+	891	WP_000971336.1	heme o synthase	-
JSU12_RS18620 (3809330)	3809330..3809641	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JSU12_RS18625 (3809625)	3809625..3810167	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JSU12_RS18630 (3810223)	3810223..3811158	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
JSU12_RS18635 (3811566)	3811566..3812930	+	1365	WP_023143167.1	MFS transporter	-
JSU12_RS18640 (3813058)	3813058..3813549	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
JSU12_RS18645 (3813717)	3813717..3814628	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T191594 WP_000227784.1 NZ_CP069677:3809625-3810167 [Escherichia coli O126:H45]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T191594 NZ_CP091836:c1960445-1960110 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT191594 WP_001297137.1 NZ_CP069677:3809330-3809641 [Escherichia coli O126:H45]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT191594 NZ_CP091836:c1960693-1960445 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3