Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3775241..3775859 Replicon chromosome
Accession NZ_CP069677
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSU12_RS18455 Protein ID WP_001291435.1
Coordinates 3775641..3775859 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSU12_RS18450 Protein ID WP_000344800.1
Coordinates 3775241..3775615 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS18440 (3770330) 3770330..3771523 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JSU12_RS18445 (3771546) 3771546..3774695 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JSU12_RS18450 (3775241) 3775241..3775615 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSU12_RS18455 (3775641) 3775641..3775859 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JSU12_RS18460 (3776030) 3776030..3776581 + 552 WP_000102578.1 maltose O-acetyltransferase -
JSU12_RS18465 (3776697) 3776697..3777167 + 471 WP_000136192.1 YlaC family protein -
JSU12_RS18470 (3777331) 3777331..3778881 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSU12_RS18475 (3778923) 3778923..3779276 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JSU12_RS18485 (3779655) 3779655..3779966 + 312 WP_000409911.1 MGMT family protein -
JSU12_RS18490 (3779997) 3779997..3780569 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191593 WP_001291435.1 NZ_CP069677:3775641-3775859 [Escherichia coli O126:H45]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191593 NZ_CP091836:c980255-980152 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191593 WP_000344800.1 NZ_CP069677:3775241-3775615 [Escherichia coli O126:H45]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191593 NZ_CP091836:980114-980385 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References