Detailed information of TA system TA191578

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 782787..783622
• Replicon: chromosome
• Accession: NZ_CP069677
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JSU12_RS03775
• Protein ID: WP_208207857.1
• Coordinates: 782787..783164 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JSU12_RS03780
• Protein ID: WP_024174331.1
• Coordinates: 783254..783622 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS03745 (778190)	778190..779167	+	978	WP_000633235.1	type II secretion system minor pseudopilin GspK	-
JSU12_RS03750 (779164)	779164..780342	+	1179	WP_000094957.1	type II secretion system protein GspL	-
JSU12_RS03755 (780344)	780344..780880	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
JSU12_RS03760 (781161)	781161..782003	-	843	WP_112025029.1	DUF4942 domain-containing protein	-
JSU12_RS03765 (782109)	782109..782285	-	177	WP_033876239.1	DUF957 domain-containing protein	-
JSU12_RS03770 (782302)	782302..782790	-	489	WP_019842393.1	DUF5983 family protein	-
JSU12_RS03775 (782787)	782787..783164	-	378	WP_208207857.1	TA system toxin CbtA family protein	Toxin
JSU12_RS03780 (783254)	783254..783622	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JSU12_RS03785 (783696)	783696..783917	-	222	WP_000692346.1	DUF987 domain-containing protein	-
JSU12_RS03790 (783986)	783986..784462	-	477	WP_001186725.1	RadC family protein	-
JSU12_RS03795 (784478)	784478..784963	-	486	WP_000214415.1	antirestriction protein	-
JSU12_RS03800 (785055)	785055..785873	-	819	WP_001234749.1	DUF932 domain-containing protein	-
JSU12_RS03805 (785985)	785985..786218	-	234	WP_001119729.1	DUF905 family protein	-
JSU12_RS03810 (786297)	786297..786752	-	456	WP_047649566.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14204.17 Da
• Isoelectric Point: 7.8050

>T191578 WP_208207857.1 NZ_CP069677:c783164-782787 [Escherichia coli O126:H45]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRAIGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T191578 NZ_CP091814:23177-23461 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT191578 WP_024174331.1 NZ_CP069677:c783622-783254 [Escherichia coli O126:H45]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT191578 NZ_CP091814:22939-23187 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA