Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 291697..292497 Replicon chromosome
Accession NZ_CP069677
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag JSU12_RS01330 Protein ID WP_000342449.1
Coordinates 291970..292497 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JSU12_RS01325 Protein ID WP_001277108.1
Coordinates 291697..291963 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS01305 (287355) 287355..288023 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JSU12_RS01310 (288016) 288016..289074 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JSU12_RS01315 (289319) 289319..290173 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JSU12_RS01320 (290444) 290444..291547 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JSU12_RS01325 (291697) 291697..291963 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
JSU12_RS01330 (291970) 291970..292497 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
JSU12_RS01335 (292494) 292494..292877 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
JSU12_RS01340 (293301) 293301..294410 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JSU12_RS01345 (294458) 294458..295384 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JSU12_RS01350 (295381) 295381..296658 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
JSU12_RS01355 (296655) 296655..297422 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T191576 WP_000342449.1 NZ_CP069677:291970-292497 [Escherichia coli O126:H45]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T191576 NZ_CP091813:c4758213-4757929 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT191576 WP_001277108.1 NZ_CP069677:291697-291963 [Escherichia coli O126:H45]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT191576 NZ_CP091813:c4758445-4758203 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References