Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 270407..270993 Replicon chromosome
Accession NZ_CP069677
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag JSU12_RS01205 Protein ID WP_042014162.1
Coordinates 270407..270637 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JSU12_RS01210 Protein ID WP_000593555.1
Coordinates 270634..270993 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS01195 (266444) 266444..269179 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
JSU12_RS01200 (269179) 269179..270303 + 1125 WP_001314210.1 ABC transporter permease -
JSU12_RS01205 (270407) 270407..270637 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
JSU12_RS01210 (270634) 270634..270993 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JSU12_RS01215 (271113) 271113..271514 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JSU12_RS01220 (271520) 271520..272326 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
JSU12_RS01225 (272323) 272323..273087 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JSU12_RS01230 (273087) 273087..273920 - 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
JSU12_RS01235 (273917) 273917..274861 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T191575 WP_042014162.1 NZ_CP069677:270407-270637 [Escherichia coli O126:H45]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T191575 NZ_CP091813:c4633729-4633196 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT191575 WP_000593555.1 NZ_CP069677:270634-270993 [Escherichia coli O126:H45]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT191575 NZ_CP091813:c4634006-4633740 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References