Detailed information of TA system TA191574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 22239..22831
• Replicon: plasmid pMUB-MIN11-MCR
• Accession: NZ_CP069669
• Organism: Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: JSU13_RS23855
• Protein ID: WP_000520549.1
• Coordinates: 22239..22487 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: JSU13_RS23860
• Protein ID: WP_000681613.1
• Coordinates: 22484..22831 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU13_RS23825 (JSU13_23810)	19116..19631	+	516	WP_001025395.1	J domain-containing protein	-
JSU13_RS23830 (JSU13_23815)	19736..20278	+	543	WP_001083906.1	hypothetical protein	-
JSU13_RS23835 (JSU13_23820)	20275..20526	+	252	WP_001230707.1	hypothetical protein	-
JSU13_RS23840 (JSU13_23825)	20513..20740	+	228	WP_000801440.1	hypothetical protein	-
JSU13_RS23845 (JSU13_23830)	21194..21493	+	300	WP_000650309.1	hypothetical protein	-
JSU13_RS23850 (JSU13_23835)	21584..21934	+	351	WP_000854259.1	hypothetical protein	-
JSU13_RS23855 (JSU13_23840)	22239..22487	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
JSU13_RS23860 (JSU13_23845)	22484..22831	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JSU13_RS23865 (JSU13_23850)	23192..23695	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
JSU13_RS23870 (JSU13_23855)	23699..24928	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
JSU13_RS23875 (JSU13_23860)	25005..25298	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
JSU13_RS23880 (JSU13_23865)	25295..25510	+	216	WP_000760375.1	EexN family lipoprotein	-
JSU13_RS23885 (JSU13_23870)	25866..26438	+	573	WP_227537772.1	lytic transglycosylase domain-containing protein	-
JSU13_RS23890 (JSU13_23875)	26499..26825	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33303	33303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T191574 WP_000520549.1 NZ_CP069669:22239-22487 [Escherichia coli O89m:H9]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T191574 NZ_CP091813:3994082-3994300 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT191574 WP_000681613.1 NZ_CP069669:22484-22831 [Escherichia coli O89m:H9]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT191574 NZ_CP091813:3993681-3994055 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1