Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 20275..20740 | Replicon | plasmid pMUB-MIN11-MCR |
| Accession | NZ_CP069669 | ||
| Organism | Escherichia coli O89m:H9 strain MIN11 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9ZUY2 |
| Locus tag | JSU13_RS23835 | Protein ID | WP_001230707.1 |
| Coordinates | 20275..20526 (+) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | M7CCF8 |
| Locus tag | JSU13_RS23840 | Protein ID | WP_000801440.1 |
| Coordinates | 20513..20740 (+) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JSU13_RS23810 (JSU13_23795) | 15756..16283 | - | 528 | WP_000577043.1 | DUF2726 domain-containing protein | - |
| JSU13_RS23815 (JSU13_23800) | 16418..16822 | - | 405 | WP_012421203.1 | hypothetical protein | - |
| JSU13_RS23820 (JSU13_23805) | 16835..17617 | - | 783 | WP_000729201.1 | replication initiation protein | - |
| JSU13_RS23825 (JSU13_23810) | 19116..19631 | + | 516 | WP_001025395.1 | J domain-containing protein | - |
| JSU13_RS23830 (JSU13_23815) | 19736..20278 | + | 543 | WP_001083906.1 | hypothetical protein | - |
| JSU13_RS23835 (JSU13_23820) | 20275..20526 | + | 252 | WP_001230707.1 | hypothetical protein | Toxin |
| JSU13_RS23840 (JSU13_23825) | 20513..20740 | + | 228 | WP_000801440.1 | hypothetical protein | Antitoxin |
| JSU13_RS23845 (JSU13_23830) | 21194..21493 | + | 300 | WP_000650309.1 | hypothetical protein | - |
| JSU13_RS23850 (JSU13_23835) | 21584..21934 | + | 351 | WP_000854259.1 | hypothetical protein | - |
| JSU13_RS23855 (JSU13_23840) | 22239..22487 | + | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | - |
| JSU13_RS23860 (JSU13_23845) | 22484..22831 | + | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| JSU13_RS23865 (JSU13_23850) | 23192..23695 | + | 504 | WP_000781813.1 | relaxosome-coupling protein TaxA | - |
| JSU13_RS23870 (JSU13_23855) | 23699..24928 | + | 1230 | WP_001329504.1 | DNA relaxase/nickase TaxC | - |
| JSU13_RS23875 (JSU13_23860) | 25005..25298 | + | 294 | WP_060598542.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| JSU13_RS23880 (JSU13_23865) | 25295..25510 | + | 216 | WP_000760375.1 | EexN family lipoprotein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mcr-1.1 | - | 1..33303 | 33303 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9751.13 Da Isoelectric Point: 8.9187
>T191573 WP_001230707.1 NZ_CP069669:20275-20526 [Escherichia coli O89m:H9]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
Download Length: 252 bp
>T191573 NZ_CP091813:c3862925-3862608 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8659.98 Da Isoelectric Point: 8.5105
>AT191573 WP_000801440.1 NZ_CP069669:20513-20740 [Escherichia coli O89m:H9]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
Download Length: 228 bp
>AT191573 NZ_CP091813:c3862615-3862328 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9ZUY2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Y6U8W9 |