Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1774..2321 Replicon plasmid pMUB-MIN11-2
Accession NZ_CP069668
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag JSU13_RS23305 Protein ID WP_001384452.1
Coordinates 2043..2321 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q4FIL6
Locus tag JSU13_RS23300 Protein ID WP_000079945.1
Coordinates 1774..2043 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS23290 (1) 1..858 + 858 WP_033803063.1 incFII family plasmid replication initiator RepA -
JSU13_RS23295 (1561) 1561..1701 + 141 WP_000829596.1 hypothetical protein -
JSU13_RS23300 (1774) 1774..2043 + 270 WP_000079945.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
JSU13_RS23305 (2043) 2043..2321 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
JSU13_RS23310 (2393) 2393..2571 + 179 Protein_4 type II toxin-antitoxin system RelE/ParE family toxin -
JSU13_RS23315 (2642) 2642..3523 + 882 WP_000005460.1 hypothetical protein -
JSU13_RS23320 (3458) 3458..3877 - 420 WP_024181414.1 hypothetical protein -
JSU13_RS23325 (3986) 3986..4336 - 351 WP_001414994.1 plasmid partitioning/stability family protein -
JSU13_RS23330 (4339) 4339..5301 - 963 WP_000959884.1 plasmid segregation protein ParM -
JSU13_RS24390 (5472) 5472..5741 + 270 WP_072126982.1 hypothetical protein -
JSU13_RS23335 (5836) 5836..6738 + 903 WP_208207793.1 DUF1281 domain-containing protein -
JSU13_RS23340 (6735) 6735..7046 + 312 WP_032144809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) - 1..74912 74912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T191572 WP_001384452.1 NZ_CP069668:2043-2321 [Escherichia coli O89m:H9]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T191572 NZ_CP091813:c2628051-2626729 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10180.52 Da        Isoelectric Point: 8.8735

>AT191572 WP_000079945.1 NZ_CP069668:1774-2043 [Escherichia coli O89m:H9]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT191572 NZ_CP091813:c2628317-2628051 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAATCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCGACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB Q4FIL6

References