Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3949109..3949685 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JSU13_RS18935 Protein ID WP_001297643.1
Coordinates 3949398..3949685 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JSU13_RS18930 Protein ID WP_000063156.1
Coordinates 3949109..3949411 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS18915 (3945705) 3945705..3947855 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
JSU13_RS18920 (3947905) 3947905..3948108 + 204 WP_000467859.1 YbdD/YjiX family protein -
JSU13_RS18925 (3948119) 3948119..3949075 + 957 WP_001297640.1 GTPase -
JSU13_RS18930 (3949109) 3949109..3949411 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JSU13_RS18935 (3949398) 3949398..3949685 - 288 WP_001297643.1 BrnT family toxin Toxin
JSU13_RS18940 (3949884) 3949884..3950581 - 698 Protein_3749 IS1 family transposase -
JSU13_RS18945 (3950638) 3950638..3950733 + 96 Protein_3750 dienelactone hydrolase family protein -
JSU13_RS18950 (3950879) 3950879..3952113 + 1235 Protein_3751 multidrug efflux MFS transporter MdtM -
JSU13_RS18955 (3952154) 3952154..3953434 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 3882935..3950387 67452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T191568 WP_001297643.1 NZ_CP069666:c3949685-3949398 [Escherichia coli O89m:H9]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T191568 NZ_CP091806:c148198-147782 [Klebsiella quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT191568 WP_000063156.1 NZ_CP069666:c3949411-3949109 [Escherichia coli O89m:H9]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT191568 NZ_CP091806:c148425-148195 [Klebsiella quasipneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References