Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3335961..3336579 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSU13_RS16005 Protein ID WP_001291435.1
Coordinates 3336361..3336579 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSU13_RS16000 Protein ID WP_000344800.1
Coordinates 3335961..3336335 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS15990 (3331050) 3331050..3332243 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JSU13_RS15995 (3332266) 3332266..3335415 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JSU13_RS16000 (3335961) 3335961..3336335 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSU13_RS16005 (3336361) 3336361..3336579 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JSU13_RS16010 (3336751) 3336751..3337302 + 552 WP_000102564.1 maltose O-acetyltransferase -
JSU13_RS16015 (3337418) 3337418..3337888 + 471 WP_000136192.1 YlaC family protein -
JSU13_RS16020 (3338052) 3338052..3339602 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSU13_RS16025 (3339644) 3339644..3339997 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JSU13_RS16035 (3340376) 3340376..3340687 + 312 WP_000409911.1 MGMT family protein -
JSU13_RS16040 (3340718) 3340718..3341290 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191564 WP_001291435.1 NZ_CP069666:3336361-3336579 [Escherichia coli O89m:H9]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191564 NZ_CP091805:4009084-4009302 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191564 WP_000344800.1 NZ_CP069666:3335961-3336335 [Escherichia coli O89m:H9]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191564 NZ_CP091805:4008683-4009057 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References