Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 819448..820102 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JSU13_RS04000 Protein ID WP_000244777.1
Coordinates 819695..820102 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSU13_RS03995 Protein ID WP_000354046.1
Coordinates 819448..819714 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS03970 (814617) 814617..815360 + 744 WP_000951964.1 SDR family oxidoreductase -
JSU13_RS03975 (815417) 815417..816850 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JSU13_RS03980 (816895) 816895..817206 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JSU13_RS03985 (817370) 817370..818029 + 660 WP_000250274.1 hemolysin III family protein -
JSU13_RS03990 (818225) 818225..819205 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JSU13_RS03995 (819448) 819448..819714 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSU13_RS04000 (819695) 819695..820102 + 408 WP_000244777.1 protein YgfX Toxin
JSU13_RS04005 (820142) 820142..820663 - 522 WP_001055874.1 flavodoxin FldB -
JSU13_RS04010 (820775) 820775..821671 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSU13_RS04015 (821696) 821696..822406 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSU13_RS04020 (822412) 822412..824145 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T191551 WP_000244777.1 NZ_CP069666:819695-820102 [Escherichia coli O89m:H9]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T191551 NZ_CP091779:c3784971-3784561 [Enterobacter mori]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCATGGCGCTCACAGTGGATGTCATTACTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTAAGCTATACGCCTCTGTGGTTATTGCTGCTGTCGTTTGTGGTTTTTGATAGCG
TGCGCAGCCAGCGCCGGATCAACGCCCGTCAGGGTGAAATCAAACTGCTGATGGATTCCCGTCTGCGCTGGCAGGGGAAA
GAGTGGGAGATCGTCGGTACACCGTGGATGCTTAGCGTTGGCATGATGCTGAGACTGCGCCAGCCTGAAGGTGGTCGTCG
CCAGCACCTGTGGCTGGCTGCCGACAGTATGGATGTTGCGGAGTGGCGAGATCTGCGCCGGATGATGTTGCAACAACCGA
CGCAGGACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT191551 WP_000354046.1 NZ_CP069666:819448-819714 [Escherichia coli O89m:H9]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT191551 NZ_CP091779:c3785218-3784952 [Enterobacter mori]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGCGAACTTGATATCTCCATCATGCCGTT
CTTTGAATATGAGTATGACAGCTTAAGCGATGACGATAAGCGTCTGTTTGTTCGCCTTCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References