Detailed information of TA system TA191547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 22239..22831
• Replicon: plasmid pMUB-MIN12-MCR
• Accession: NZ_CP069661
• Organism: Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: JSU14_RS25460
• Protein ID: WP_000520549.1
• Coordinates: 22239..22487 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: JSU14_RS25465
• Protein ID: WP_000681613.1
• Coordinates: 22484..22831 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU14_RS25430 (JSU14_25470)	19116..19631	+	516	WP_001025395.1	J domain-containing protein	-
JSU14_RS25435 (JSU14_25475)	19736..20278	+	543	WP_001083906.1	hypothetical protein	-
JSU14_RS25440 (JSU14_25480)	20275..20526	+	252	WP_001230707.1	hypothetical protein	-
JSU14_RS25445 (JSU14_25485)	20513..20740	+	228	WP_000801440.1	hypothetical protein	-
JSU14_RS25450 (JSU14_25490)	21194..21493	+	300	WP_000650309.1	hypothetical protein	-
JSU14_RS25455 (JSU14_25495)	21584..21934	+	351	WP_000854259.1	hypothetical protein	-
JSU14_RS25460 (JSU14_25500)	22239..22487	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
JSU14_RS25465 (JSU14_25505)	22484..22831	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JSU14_RS25470 (JSU14_25510)	23192..23695	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
JSU14_RS25475 (JSU14_25515)	23699..24928	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
JSU14_RS25480 (JSU14_25520)	25005..25298	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
JSU14_RS25485 (JSU14_25525)	25295..25510	+	216	WP_000760375.1	EexN family lipoprotein	-
JSU14_RS25490 (JSU14_25530)	25866..26438	+	573	WP_227537772.1	lytic transglycosylase domain-containing protein	-
JSU14_RS25495 (JSU14_25535)	26499..26825	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33303	33303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T191547 WP_000520549.1 NZ_CP069661:22239-22487 [Escherichia coli O89m:H10]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T191547 NZ_CP091779:488311-488595 [Enterobacter mori]
ATGACTTATGAACTGGCGTTTGATCCTAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGGAGACTGTAAAAGACCAGTTTAA
GAAGAAACTTGCGGATGTGTTGGTGAACCCAAGGGTCGAATCGGCCCGCCTGCATGGTCTACCGGATTGCTACAAAATAA
AACTCAGATCGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAATGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT191547 WP_000681613.1 NZ_CP069661:22484-22831 [Escherichia coli O89m:H10]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT191547 NZ_CP091779:488079-488321 [Enterobacter mori]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTACGCCGTGCTGGAAAAACTCAATATCACCCC
TACGGAAGCGGTGCGATTACTGTTTCAGTACGTAGCTGAAACAGGGCGCATGCCCGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACGGTCCGGGAGCGGCTGGCCAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1