Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1940..2499 Replicon plasmid pMUB-MIN12-2
Accession NZ_CP069659
Organism Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag JSU14_RS24430 Protein ID WP_000421257.1
Coordinates 2224..2499 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JSU14_RS24425 Protein ID WP_001178089.1
Coordinates 1940..2224 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU14_RS24420 (JSU14_24450) 1..1032 + 1032 WP_000907880.1 plasmid replication initiator RepA -
JSU14_RS24425 (JSU14_24455) 1940..2224 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JSU14_RS24430 (JSU14_24460) 2224..2499 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JSU14_RS24435 (JSU14_24465) 2605..2808 + 204 Protein_3 hypothetical protein -
JSU14_RS24440 (JSU14_24470) 3077..3343 - 267 WP_000627831.1 hypothetical protein -
JSU14_RS24450 (JSU14_24480) 3581..4183 - 603 WP_000517694.1 ProQ/FINO family protein -
JSU14_RS24455 (JSU14_24485) 4537..5883 + 1347 WP_001132025.1 protein YagA -
JSU14_RS24460 (JSU14_24490) 6426..6728 - 303 WP_001245884.1 hypothetical protein -
JSU14_RS24465 (JSU14_24495) 6725..7351 - 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / aph(6)-Id / blaTEM-1B / aph(3'')-Ib / sul2 - 1..95526 95526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T191544 WP_000421257.1 NZ_CP069659:2224-2499 [Escherichia coli O89m:H10]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T191544 NZ_CP091774:43609-44007 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT191544 WP_001178089.1 NZ_CP069659:1940-2224 [Escherichia coli O89m:H10]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT191544 NZ_CP091774:43382-43609 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References