Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4165508..4166084 Replicon chromosome
Accession NZ_CP069657
Organism Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JSU14_RS19970 Protein ID WP_001297643.1
Coordinates 4165797..4166084 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JSU14_RS19965 Protein ID WP_000063156.1
Coordinates 4165508..4165810 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU14_RS19950 (4162104) 4162104..4164254 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
JSU14_RS19955 (4164304) 4164304..4164507 + 204 WP_000467859.1 YbdD/YjiX family protein -
JSU14_RS19960 (4164518) 4164518..4165474 + 957 WP_001297640.1 GTPase -
JSU14_RS19965 (4165508) 4165508..4165810 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JSU14_RS19970 (4165797) 4165797..4166084 - 288 WP_001297643.1 BrnT family toxin Toxin
JSU14_RS19975 (4166283) 4166283..4166980 - 698 Protein_3955 IS1 family transposase -
JSU14_RS19980 (4167037) 4167037..4167132 + 96 Protein_3956 dienelactone hydrolase family protein -
JSU14_RS19985 (4167278) 4167278..4168510 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
JSU14_RS19990 (4168551) 4168551..4169831 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 4097602..4166786 69184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T191539 WP_001297643.1 NZ_CP069657:c4166084-4165797 [Escherichia coli O89m:H10]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T191539 NZ_CP091773:2691269-2691376 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGTGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT191539 WP_000063156.1 NZ_CP069657:c4165810-4165508 [Escherichia coli O89m:H10]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT191539 NZ_CP091773:c2691216-2691155 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References