Detailed information of TA system TA191535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2530417..2531055
• Replicon: chromosome
• Accession: NZ_CP069657
• Organism: Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: JSU14_RS12040
• Protein ID: WP_001447010.1
• Coordinates: 2530879..2531055 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JSU14_RS12035
• Protein ID: WP_001270286.1
• Coordinates: 2530417..2530833 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU14_RS12015 (2525569)	2525569..2526510	-	942	WP_001251304.1	ABC transporter permease	-
JSU14_RS12020 (2526511)	2526511..2527524	-	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
JSU14_RS12025 (2527542)	2527542..2528687	-	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
JSU14_RS12030 (2528932)	2528932..2530338	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JSU14_RS12035 (2530417)	2530417..2530833	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JSU14_RS12040 (2530879)	2530879..2531055	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JSU14_RS12045 (2531277)	2531277..2531507	+	231	WP_000494244.1	YncJ family protein	-
JSU14_RS12050 (2531599)	2531599..2533560	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JSU14_RS12055 (2533633)	2533633..2534169	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JSU14_RS12060 (2534261)	2534261..2535436	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T191535 WP_001447010.1 NZ_CP069657:c2531055-2530879 [Escherichia coli O89m:H10]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T191535 NZ_CP091773:1882550-1882804 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAATGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT191535 WP_001270286.1 NZ_CP069657:c2530833-2530417 [Escherichia coli O89m:H10]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT191535 NZ_CP091773:1882302-1882553 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8