Detailed information of TA system TA191524

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1173559..1174394
• Replicon: chromosome
• Accession: NZ_CP069657
• Organism: Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JSU14_RS05670
• Protein ID: WP_068862752.1
• Coordinates: 1173559..1173936 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J4HRK6
• Locus tag: JSU14_RS05675
• Protein ID: WP_001354276.1
• Coordinates: 1174026..1174394 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU14_RS05645 (1168949)	1168949..1171201	-	2253	Protein_1118	alpha-amylase family glycosyl hydrolase	-
JSU14_RS05655 (1171935)	1171935..1172780	-	846	WP_068862747.1	DUF4942 domain-containing protein	-
JSU14_RS05660 (1172865)	1172865..1173062	-	198	WP_029396577.1	DUF957 domain-containing protein	-
JSU14_RS05665 (1173074)	1173074..1173562	-	489	WP_068862750.1	DUF5983 family protein	-
JSU14_RS05670 (1173559)	1173559..1173936	-	378	WP_068862752.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JSU14_RS05675 (1174026)	1174026..1174394	-	369	WP_001354276.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JSU14_RS05680 (1174444)	1174444..1175088	-	645	WP_085313957.1	antitoxin of toxin-antitoxin stability system	-
JSU14_RS05685 (1175103)	1175103..1175324	-	222	WP_000692353.1	DUF987 domain-containing protein	-
JSU14_RS05690 (1175393)	1175393..1175869	-	477	WP_001384029.1	RadC family protein	-
JSU14_RS05695 (1175884)	1175884..1176369	-	486	WP_085313958.1	antirestriction protein	-
JSU14_RS05700 (1176461)	1176461..1177279	-	819	WP_134234185.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1170164..1205134	34970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13911.90 Da
• Isoelectric Point: 7.9085

>T191524 WP_068862752.1 NZ_CP069657:c1173936-1173559 [Escherichia coli O89m:H10]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T191524 NZ_CP091770:c627899-627618 [Helicobacter pylori]
ATGTTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACGAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCGCTCCAACAAAAGTATTGCGACCATGCCTTAAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTATT
TTATTGCGTCTAGACACTCACAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13649.47 Da
• Isoelectric Point: 7.0261

>AT191524 WP_001354276.1 NZ_CP069657:c1174394-1174026 [Escherichia coli O89m:H10]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT191524 NZ_CP091770:c628206-627892 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGCTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4HRK6