Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 911127..911781 Replicon chromosome
Accession NZ_CP069657
Organism Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JSU14_RS04415 Protein ID WP_000244777.1
Coordinates 911374..911781 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSU14_RS04410 Protein ID WP_000354046.1
Coordinates 911127..911393 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU14_RS04385 (906296) 906296..907039 + 744 WP_000951964.1 SDR family oxidoreductase -
JSU14_RS04390 (907096) 907096..908529 - 1434 WP_001446971.1 6-phospho-beta-glucosidase BglA -
JSU14_RS04395 (908574) 908574..908885 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JSU14_RS04400 (909049) 909049..909708 + 660 WP_000250274.1 hemolysin III family protein -
JSU14_RS04405 (909904) 909904..910884 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JSU14_RS04410 (911127) 911127..911393 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSU14_RS04415 (911374) 911374..911781 + 408 WP_000244777.1 protein YgfX Toxin
JSU14_RS04420 (911821) 911821..912342 - 522 WP_001055874.1 flavodoxin FldB -
JSU14_RS04425 (912454) 912454..913350 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSU14_RS04430 (913375) 913375..914085 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSU14_RS04435 (914091) 914091..915824 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T191522 WP_000244777.1 NZ_CP069657:911374-911781 [Escherichia coli O89m:H10]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T191522 NZ_CP091767:2316655-2317392 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT191522 WP_000354046.1 NZ_CP069657:911127-911393 [Escherichia coli O89m:H10]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT191522 NZ_CP091767:2317505-2317630 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References