Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 28223..28688 | Replicon | plasmid pMUB-MIN14-MCR |
| Accession | NZ_CP069650 | ||
| Organism | Escherichia coli O7:H4 strain MIN14 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9ZUY2 |
| Locus tag | JSU15_RS24415 | Protein ID | WP_001230707.1 |
| Coordinates | 28437..28688 (-) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | M7CCF8 |
| Locus tag | JSU15_RS24410 | Protein ID | WP_000801440.1 |
| Coordinates | 28223..28450 (-) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JSU15_RS24370 (JSU15_24370) | 23453..23668 | - | 216 | WP_000760375.1 | EexN family lipoprotein | - |
| JSU15_RS24375 (JSU15_24375) | 23665..23958 | - | 294 | WP_060598542.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| JSU15_RS24380 (JSU15_24380) | 24035..25264 | - | 1230 | WP_001329504.1 | DNA relaxase/nickase TaxC | - |
| JSU15_RS24385 (JSU15_24385) | 25268..25771 | - | 504 | WP_000781813.1 | relaxosome-coupling protein TaxA | - |
| JSU15_RS24390 (JSU15_24390) | 26132..26479 | - | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| JSU15_RS24395 (JSU15_24395) | 26476..26724 | - | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | - |
| JSU15_RS24400 (JSU15_24400) | 27029..27379 | - | 351 | WP_000854259.1 | hypothetical protein | - |
| JSU15_RS24405 (JSU15_24405) | 27470..27769 | - | 300 | WP_000650309.1 | hypothetical protein | - |
| JSU15_RS24410 (JSU15_24410) | 28223..28450 | - | 228 | WP_000801440.1 | hypothetical protein | Antitoxin |
| JSU15_RS24415 (JSU15_24415) | 28437..28688 | - | 252 | WP_001230707.1 | hypothetical protein | Toxin |
| JSU15_RS24420 (JSU15_24420) | 28685..29227 | - | 543 | WP_001083906.1 | hypothetical protein | - |
| JSU15_RS24425 (JSU15_24425) | 29332..29847 | - | 516 | WP_001025395.1 | J domain-containing protein | - |
| JSU15_RS24430 (JSU15_24430) | 31259..31960 | + | 702 | WP_251361261.1 | replication initiation protein | - |
| JSU15_RS24435 (JSU15_24435) | 32135..32539 | + | 405 | WP_208232115.1 | hypothetical protein | - |
| JSU15_RS24440 (JSU15_24440) | 32674..33201 | + | 528 | WP_000577043.1 | DUF2726 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mcr-1.1 | - | 1..33290 | 33290 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9751.13 Da Isoelectric Point: 8.9187
>T191517 WP_001230707.1 NZ_CP069650:c28688-28437 [Escherichia coli O7:H4]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
Download Length: 252 bp
>T191517 NZ_CP091762:241947-242297 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8659.98 Da Isoelectric Point: 8.5105
>AT191517 WP_000801440.1 NZ_CP069650:c28450-28223 [Escherichia coli O7:H4]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
Download Length: 228 bp
>AT191517 NZ_CP091762:241655-241942 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9ZUY2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Y6U8W9 |