Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 26132..26724 Replicon plasmid pMUB-MIN14-MCR
Accession NZ_CP069650
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag JSU15_RS24395 Protein ID WP_000520549.1
Coordinates 26476..26724 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag JSU15_RS24390 Protein ID WP_000681613.1
Coordinates 26132..26479 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS24360 (JSU15_24360) 22138..22464 - 327 WP_000008647.1 TrbC/VirB2 family protein -
JSU15_RS24365 (JSU15_24365) 22525..23097 - 573 WP_227537772.1 lytic transglycosylase domain-containing protein -
JSU15_RS24370 (JSU15_24370) 23453..23668 - 216 WP_000760375.1 EexN family lipoprotein -
JSU15_RS24375 (JSU15_24375) 23665..23958 - 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
JSU15_RS24380 (JSU15_24380) 24035..25264 - 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
JSU15_RS24385 (JSU15_24385) 25268..25771 - 504 WP_000781813.1 relaxosome-coupling protein TaxA -
JSU15_RS24390 (JSU15_24390) 26132..26479 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JSU15_RS24395 (JSU15_24395) 26476..26724 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
JSU15_RS24400 (JSU15_24400) 27029..27379 - 351 WP_000854259.1 hypothetical protein -
JSU15_RS24405 (JSU15_24405) 27470..27769 - 300 WP_000650309.1 hypothetical protein -
JSU15_RS24410 (JSU15_24410) 28223..28450 - 228 WP_000801440.1 hypothetical protein -
JSU15_RS24415 (JSU15_24415) 28437..28688 - 252 WP_001230707.1 hypothetical protein -
JSU15_RS24420 (JSU15_24420) 28685..29227 - 543 WP_001083906.1 hypothetical protein -
JSU15_RS24425 (JSU15_24425) 29332..29847 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33290 33290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T191516 WP_000520549.1 NZ_CP069650:c26724-26476 [Escherichia coli O7:H4]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T191516 NZ_CP091758:39708-39857 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT191516 WP_000681613.1 NZ_CP069650:c26479-26132 [Escherichia coli O7:H4]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT191516 NZ_CP091758:c39664-39603 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References