Detailed information of TA system TA191515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 59345..59989
• Replicon: plasmid pMUB-MIN14-1
• Accession: NZ_CP069647
• Organism: Escherichia coli O7:H4 strain MIN14

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1C0Y1A0
• Locus tag: JSU15_RS23430
• Protein ID: WP_029701816.1
• Coordinates: 59807..59989 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JSU15_RS23425
• Protein ID: WP_029701815.1
• Coordinates: 59345..59776 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU15_RS23400 (JSU15_23400)	54389..54712	+	324	WP_251361242.1	hypothetical protein	-
JSU15_RS23410 (JSU15_23410)	54824..57391	+	2568	WP_251361243.1	head protein	-
JSU15_RS23415 (JSU15_23415)	57388..57756	+	369	WP_016262342.1	hypothetical protein	-
JSU15_RS23420 (JSU15_23420)	57848..59233	+	1386	WP_068862841.1	hypothetical protein	-
JSU15_RS23425 (JSU15_23425)	59345..59776	-	432	WP_029701815.1	hypothetical protein	Antitoxin
JSU15_RS23430 (JSU15_23430)	59807..59989	-	183	WP_029701816.1	type II toxin-antitoxin system HicA family toxin	Toxin
JSU15_RS23435 (JSU15_23435)	60222..61397	+	1176	WP_251361244.1	hypothetical protein	-
JSU15_RS24955	61394..61906	+	513	WP_251361245.1	hypothetical protein	-
JSU15_RS23440 (JSU15_23440)	61920..62918	+	999	WP_021551762.1	hypothetical protein	-
JSU15_RS23445 (JSU15_23445)	63118..64080	+	963	WP_068862843.1	lytic replication protein	-
JSU15_RS23450 (JSU15_23450)	64497..64721	+	225	WP_000245715.1	host cell division inhibitor Icd-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89674	89674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6850.97 Da
• Isoelectric Point: 10.9041

>T191515 WP_029701816.1 NZ_CP069647:c59989-59807 [Escherichia coli O7:H4]
VKYSEFKRWLIQQGAEFRKAPGGGSHQKVNLNGKRSVFPDHGSKEMPEPLRKKIMKDLGL

Nucleotide

• Length: 183 bp

>T191515 NZ_CP091758:c18723-18337 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15656.01 Da
• Isoelectric Point: 5.2844

>AT191515 WP_029701815.1 NZ_CP069647:c59776-59345 [Escherichia coli O7:H4]
MFNYPVKLERDDKTGAYVVSCRDLPLFNSVGDSVEEALLEAGYGLVAAVAIEIEERRPVPTGSEPKEGEYVVSLPVLPAM
KAALHNAMIETGTRKAELARKLGKNGTQIDRLLDVEHSSKVETVELALHQLNKNIVVSVTPMR

Nucleotide

• Length: 432 bp

>AT191515 NZ_CP091758:c18944-18720 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCTGT
TGAGATTCAGCGCCGTAAAGGTGGCGCGGCTGTACTAGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA

Structures

Toxin

• AlphaFold DB: A0A1C0Y1A0