Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3962023..3962677 Replicon chromosome
Accession NZ_CP069646
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag JSU15_RS19250 Protein ID WP_208231903.1
Coordinates 3962270..3962677 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSU15_RS19245 Protein ID WP_000354046.1
Coordinates 3962023..3962289 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS19220 (3957192) 3957192..3957935 + 744 WP_000951948.1 SDR family oxidoreductase -
JSU15_RS19225 (3957992) 3957992..3959425 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
JSU15_RS19230 (3959470) 3959470..3959781 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JSU15_RS19235 (3959945) 3959945..3960604 + 660 WP_000250274.1 hemolysin III family protein -
JSU15_RS19240 (3960800) 3960800..3961780 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JSU15_RS19245 (3962023) 3962023..3962289 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSU15_RS19250 (3962270) 3962270..3962677 + 408 WP_208231903.1 protein YgfX Toxin
JSU15_RS19255 (3962717) 3962717..3963238 - 522 WP_001055874.1 flavodoxin FldB -
JSU15_RS19260 (3963350) 3963350..3964246 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSU15_RS19265 (3964271) 3964271..3964981 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSU15_RS19270 (3964987) 3964987..3966720 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15980.88 Da        Isoelectric Point: 11.2669

>T191513 WP_208231903.1 NZ_CP069646:3962270-3962677 [Escherichia coli O7:H4]
VVLWQSDLRVSWRAQWLSLLIHGMVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T191513 NZ_CP091757:256189-256605 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT191513 WP_000354046.1 NZ_CP069646:3962023-3962289 [Escherichia coli O7:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT191513 NZ_CP091757:255962-256192 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References