Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2520289..2520786 Replicon chromosome
Accession NZ_CP069646
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JSU15_RS12340 Protein ID WP_001173343.1
Coordinates 2520289..2520462 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JSU15_RS12345 Protein ID WP_000398619.1
Coordinates 2520490..2520786 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS12330 (2516959) 2516959..2518416 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
JSU15_RS12335 (2518653) 2518653..2520170 + 1518 WP_001295090.1 lysine--tRNA ligase -
JSU15_RS12340 (2520289) 2520289..2520462 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JSU15_RS12345 (2520490) 2520490..2520786 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JSU15_RS12350 (2521013) 2521013..2521285 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
JSU15_RS12355 (2521297) 2521297..2521527 - 231 WP_001070602.1 4Fe-4S mono-cluster protein YjdI -
JSU15_RS12360 (2521708) 2521708..2523339 + 1632 WP_001216477.1 sensor histidine kinase -
JSU15_RS12365 (2523336) 2523336..2524055 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papC 2465892..2520786 54894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T191508 WP_001173343.1 NZ_CP069646:c2520462-2520289 [Escherichia coli O7:H4]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T191508 NZ_CP091756:c3889574-3889176 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT191508 WP_000398619.1 NZ_CP069646:c2520786-2520490 [Escherichia coli O7:H4]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT191508 NZ_CP091756:c3889870-3889577 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References