Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1751407..1752244 Replicon chromosome
Accession NZ_CP069646
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag JSU15_RS08665 Protein ID WP_000227786.1
Coordinates 1751702..1752244 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JSU15_RS08660 Protein ID WP_001297137.1
Coordinates 1751407..1751718 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS08635 (1746427) 1746427..1747374 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
JSU15_RS08640 (1747396) 1747396..1749387 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JSU15_RS08645 (1749377) 1749377..1749991 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JSU15_RS08650 (1749991) 1749991..1750320 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JSU15_RS08655 (1750332) 1750332..1751222 + 891 WP_000971336.1 heme o synthase -
JSU15_RS08660 (1751407) 1751407..1751718 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JSU15_RS08665 (1751702) 1751702..1752244 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
JSU15_RS08670 (1752300) 1752300..1753235 - 936 WP_001297127.1 tetratricopeptide repeat protein -
JSU15_RS08675 (1753652) 1753652..1755016 + 1365 WP_001000978.1 MFS transporter -
JSU15_RS08680 (1755144) 1755144..1755635 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JSU15_RS08685 (1755803) 1755803..1756714 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T191503 WP_000227786.1 NZ_CP069646:1751702-1752244 [Escherichia coli O7:H4]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T191503 NZ_CP091756:2427775-2428062 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT191503 WP_001297137.1 NZ_CP069646:1751407-1751718 [Escherichia coli O7:H4]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT191503 NZ_CP091756:2427536-2427775 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References