Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 718776..719414 Replicon chromosome
Accession NZ_CP069646
Organism Escherichia coli O7:H4 strain MIN14

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JSU15_RS03620 Protein ID WP_000813794.1
Coordinates 719238..719414 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JSU15_RS03615 Protein ID WP_001270286.1
Coordinates 718776..719192 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU15_RS03595 (713928) 713928..714869 - 942 WP_001251313.1 ABC transporter permease -
JSU15_RS03600 (714870) 714870..715883 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JSU15_RS03605 (715901) 715901..717046 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JSU15_RS03610 (717291) 717291..718697 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
JSU15_RS03615 (718776) 718776..719192 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JSU15_RS03620 (719238) 719238..719414 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JSU15_RS03625 (719636) 719636..719866 + 231 WP_000494244.1 YncJ family protein -
JSU15_RS03630 (719958) 719958..721919 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JSU15_RS03635 (721992) 721992..722528 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JSU15_RS03640 (722620) 722620..723795 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T191501 WP_000813794.1 NZ_CP069646:c719414-719238 [Escherichia coli O7:H4]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T191501 NZ_CP091756:1911622-1911876 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT191501 WP_001270286.1 NZ_CP069646:c719192-718776 [Escherichia coli O7:H4]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT191501 NZ_CP091756:1911374-1911625 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References