Detailed information of TA system TA191500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 595756..597335
• Replicon: chromosome
• Accession: NZ_CP069646
• Organism: Escherichia coli O7:H4 strain MIN14

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JSU15_RS03110
• Protein ID: WP_208232071.1
• Coordinates: 596022..597335 (+)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JSU15_RS03105
• Protein ID: WP_001301023.1
• Coordinates: 595756..596022 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU15_RS03080 (590931)	590931..591221	-	291	WP_000558527.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
JSU15_RS03085 (591245)	591245..592120	-	876	WP_000774188.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
JSU15_RS03090 (592147)	592147..593169	-	1023	WP_000172488.1	autoinducer 2 ABC transporter substrate-binding protein LsrB	-
JSU15_RS03095 (593181)	593181..593957	-	777	Protein_612	histidine kinase	-
JSU15_RS03100 (593953)	593953..595533	+	1581	Protein_613	autotransporter outer membrane beta-barrel domain-containing protein	-
JSU15_RS03105 (595756)	595756..596022	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JSU15_RS03110 (596022)	596022..597335	+	1314	WP_208232071.1	type II toxin-antitoxin system HipA family toxin	Toxin
JSU15_RS03115 (597585)	597585..598985	+	1401	WP_001388361.1	site-specific integrase	-
JSU15_RS03120 (598969)	598969..600519	+	1551	WP_000086929.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 48960.53 Da
• Isoelectric Point: 8.8764

>T191500 WP_208232071.1 NZ_CP069646:596022-597335 [Escherichia coli O7:H4]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPIIKAGNVRALAVERFDRRWNAE
RTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGG
SYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIPAAL
DNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1314 bp

>T191500 NZ_CP091756:1911374-1911625 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT191500 WP_001301023.1 NZ_CP069646:595756-596022 [Escherichia coli O7:H4]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT191500 NZ_CP091756:1911622-1911876 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA