Detailed information of TA system TA191491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1559..2075
• Replicon: plasmid pMUT2
• Accession: NZ_CP069643
• Organism: Escherichia coli strain SSGNw2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JSQ95_RS24175
• Protein ID: WP_001554709.1
• Coordinates: 1791..2075 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8H9DQ25
• Locus tag: JSQ95_RS24170
• Protein ID: WP_001554710.1
• Coordinates: 1559..1801 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ95_RS24160 (JSQ95_23920)	1..5514	-	5514	WP_024199421.1	anti-phage protein KwaA	-
JSQ95_RS24165 (JSQ95_23925)	398..1294	-	897	WP_024199422.1	replication initiation protein	-
JSQ95_RS24170 (JSQ95_23930)	1559..1801	+	243	WP_001554710.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JSQ95_RS24175 (JSQ95_23935)	1791..2075	+	285	WP_001554709.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JSQ95_RS24180 (JSQ95_23940)	2120..2344	-	225	WP_223938297.1	MbeD family mobilization/exclusion protein	-
JSQ95_RS24185	2955..3419	-	465	Protein_5	relaxase/mobilization nuclease domain-containing protein	-
JSQ95_RS24190 (JSQ95_23950)	3577..3903	-	327	WP_001554615.1	MobC family plasmid mobilization relaxosome protein	-
JSQ95_RS24195 (JSQ95_23955)	4130..5272	-	1143	WP_001704287.1	DUF4868 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5514	5514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11265.07 Da
• Isoelectric Point: 10.4971

>T191491 WP_001554709.1 NZ_CP069643:1791-2075 [Escherichia coli]
MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLRELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGK
RERSAAYDAADKRL

Nucleotide

• Length: 285 bp

>T191491 NZ_CP091752:c5611177-5610803 [Klebsiella grimontii]
ATGATTAAAGGCCCGGCGCTGTTTGATACTAATATTCTCATCGATTTATTCAGCGGTCGCGTTGAAGCGAAACAGGTTCT
GGAGGCTTATCCGCCACAGAATGCGATTAGCCTCATCACGTGGACGGAAGTAATGGTGGGGGCGAAAAAGTATCATCAGG
AACACCGCACGCGTATTGCACTGAGCGCGTTTAATATTATCGATATTTCACAGGATATTGCTGAACGAAGCGTCAATCTC
AGGAAGGAGTATGGGATGAAGCTGCCGGATGCCATCATACTGGCGACAGCGCAAATACATCGTTATGAACTGGTCACGCG
CAATACCAAAGATTTTTTCGGGATACCCGGAGTGATTACGCCTTATCATTTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8958.21 Da
• Isoelectric Point: 4.2821

>AT191491 WP_001554710.1 NZ_CP069643:1559-1801 [Escherichia coli]
MATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRLPFQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL

Nucleotide

• Length: 243 bp

>AT191491 NZ_CP091752:c5611413-5611174 [Klebsiella grimontii]
ATGATGACGGGAACGAATATGGGCAGAATACTACTCGACTTATCGGATGACGTTATTAAACGTCTTGAAGACCTCAAACA
GCAGCGCAATCTTCCTCGAGCGGAACTGTTACGCGAGGCGGTTGAACAGTACCTGGAAAGACAAGCTGACACCACTATTT
CCAATGCGCTGGGGATTTGGCAAGGCTGTGAGGAAGATGGAGTTGAATTCGAAAGGAAACTCCGCGAGGAGTGGCAATGA