Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4039437..4040055 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSQ94_RS19175 Protein ID WP_001291435.1
Coordinates 4039437..4039655 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSQ94_RS19180 Protein ID WP_000344800.1
Coordinates 4039681..4040055 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS19140 (4034725) 4034725..4035297 + 573 WP_000779831.1 YbaY family lipoprotein -
JSQ94_RS19145 (4035328) 4035328..4035639 - 312 WP_000409908.1 MGMT family protein -
JSQ94_RS19155 (4036018) 4036018..4036371 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JSQ94_RS19160 (4036413) 4036413..4037963 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSQ94_RS19165 (4038127) 4038127..4038597 - 471 WP_001304825.1 YlaC family protein -
JSQ94_RS19170 (4038713) 4038713..4039264 - 552 WP_000102539.1 maltose O-acetyltransferase -
JSQ94_RS19175 (4039437) 4039437..4039655 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JSQ94_RS19180 (4039681) 4039681..4040055 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSQ94_RS19185 (4040600) 4040600..4043749 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
JSQ94_RS19190 (4043772) 4043772..4044965 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191381 WP_001291435.1 NZ_CP069630:c4039655-4039437 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191381 NZ_CP091698:c10035-9883 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191381 WP_000344800.1 NZ_CP069630:c4040055-4039681 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191381 NZ_CP091698:10087-10147 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References