Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3547594..3548144 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JSQ94_RS16865 Protein ID WP_001160966.1
Coordinates 3547830..3548144 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JSQ94_RS16860 Protein ID WP_000125566.1
Coordinates 3547594..3547827 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS16835 (3542667) 3542667..3542954 + 288 WP_000203741.1 ferredoxin-like protein FixX -
JSQ94_RS16840 (3543013) 3543013..3544344 + 1332 WP_001183207.1 MFS transporter -
JSQ94_RS16845 (3544452) 3544452..3544982 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JSQ94_RS16850 (3544975) 3544975..3546837 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
JSQ94_RS16855 (3547029) 3547029..3547508 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
JSQ94_RS16860 (3547594) 3547594..3547827 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JSQ94_RS16865 (3547830) 3547830..3548144 + 315 WP_001160966.1 CcdB family protein Toxin
JSQ94_RS16870 (3548141) 3548141..3548989 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JSQ94_RS16875 (3548996) 3548996..3549373 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JSQ94_RS16880 (3549376) 3549376..3550197 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JSQ94_RS16885 (3550194) 3550194..3551183 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JSQ94_RS16890 (3551183) 3551183..3552469 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T191379 WP_001160966.1 NZ_CP069630:3547830-3548144 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T191379 NZ_CP091692:20581-20997 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT191379 WP_000125566.1 NZ_CP069630:3547594-3547827 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT191379 NZ_CP091692:20354-20584 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References