Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3442280..3442856 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag JSQ94_RS16345 Protein ID WP_001295743.1
Coordinates 3442280..3442567 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag JSQ94_RS16350 Protein ID WP_000063148.1
Coordinates 3442554..3442856 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS16330 (3437940) 3437940..3439244 - 1305 WP_000535012.1 restriction endonuclease subunit S -
JSQ94_RS16335 (3439234) 3439234..3440853 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
JSQ94_RS16340 (3440917) 3440917..3442083 - 1167 WP_000800831.1 restriction endonuclease -
JSQ94_RS16345 (3442280) 3442280..3442567 + 288 WP_001295743.1 BrnT family toxin Toxin
JSQ94_RS16350 (3442554) 3442554..3442856 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
JSQ94_RS16355 (3442890) 3442890..3443846 - 957 WP_001295745.1 GTPase -
JSQ94_RS16360 (3443857) 3443857..3444060 - 204 WP_000467859.1 YbdD/YjiX family protein -
JSQ94_RS16365 (3444110) 3444110..3446260 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T191376 WP_001295743.1 NZ_CP069630:3442280-3442567 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T191376 NZ_CP091691:4561282-4561689 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT191376 WP_000063148.1 NZ_CP069630:3442554-3442856 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT191376 NZ_CP091691:4561035-4561301 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References