Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3547593..3548143 Replicon chromosome
Accession NZ_CP069627
Organism Escherichia coli strain ESM1.4

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JSQ89_RS16865 Protein ID WP_001160966.1
Coordinates 3547829..3548143 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JSQ89_RS16860 Protein ID WP_000125566.1
Coordinates 3547593..3547826 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ89_RS16835 (3542666) 3542666..3542953 + 288 WP_000203741.1 ferredoxin-like protein FixX -
JSQ89_RS16840 (3543012) 3543012..3544343 + 1332 WP_001183207.1 MFS transporter -
JSQ89_RS16845 (3544451) 3544451..3544981 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JSQ89_RS16850 (3544974) 3544974..3546836 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
JSQ89_RS16855 (3547028) 3547028..3547507 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
JSQ89_RS16860 (3547593) 3547593..3547826 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JSQ89_RS16865 (3547829) 3547829..3548143 + 315 WP_001160966.1 CcdB family protein Toxin
JSQ89_RS16870 (3548140) 3548140..3548988 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JSQ89_RS16875 (3548995) 3548995..3549372 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JSQ89_RS16880 (3549375) 3549375..3550196 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JSQ89_RS16885 (3550193) 3550193..3551182 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JSQ89_RS16890 (3551182) 3551182..3552468 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T191352 WP_001160966.1 NZ_CP069627:3547829-3548143 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T191352 NZ_CP091682:4928537-4928848 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT191352 WP_000125566.1 NZ_CP069627:3547593-3547826 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT191352 NZ_CP091682:4928849-4929139 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References