Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1660733..1661387 Replicon chromosome
Accession NZ_CP069624
Organism Escherichia coli strain PBP8

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JSQ93_RS07750 Protein ID WP_000244781.1
Coordinates 1660733..1661140 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSQ93_RS07755 Protein ID WP_000354046.1
Coordinates 1661121..1661387 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ93_RS07730 (1656690) 1656690..1658423 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
JSQ93_RS07735 (1658429) 1658429..1659139 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSQ93_RS07740 (1659164) 1659164..1660060 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSQ93_RS07745 (1660172) 1660172..1660693 + 522 WP_001055874.1 flavodoxin FldB -
JSQ93_RS07750 (1660733) 1660733..1661140 - 408 WP_000244781.1 protein YgfX Toxin
JSQ93_RS07755 (1661121) 1661121..1661387 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSQ93_RS07760 (1661630) 1661630..1662610 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
JSQ93_RS07765 (1662687) 1662687..1663346 - 660 WP_000250274.1 hemolysin III family protein -
JSQ93_RS07770 (1663510) 1663510..1663821 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
JSQ93_RS07775 (1663866) 1663866..1665299 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T191316 WP_000244781.1 NZ_CP069624:c1661140-1660733 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T191316 NZ_CP091677:c1943400-1943206 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTATTCATGTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT191316 WP_000354046.1 NZ_CP069624:c1661387-1661121 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT191316 NZ_CP091677:1943029-1943207 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTTGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References