Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4039436..4040054 Replicon chromosome
Accession NZ_CP069621
Organism Escherichia coli strain PBP5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSQ88_RS19175 Protein ID WP_001291435.1
Coordinates 4039436..4039654 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSQ88_RS19180 Protein ID WP_000344800.1
Coordinates 4039680..4040054 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ88_RS19140 (4034724) 4034724..4035296 + 573 WP_000779831.1 YbaY family lipoprotein -
JSQ88_RS19145 (4035327) 4035327..4035638 - 312 WP_000409908.1 MGMT family protein -
JSQ88_RS19155 (4036017) 4036017..4036370 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JSQ88_RS19160 (4036412) 4036412..4037962 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSQ88_RS19165 (4038126) 4038126..4038596 - 471 WP_001304825.1 YlaC family protein -
JSQ88_RS19170 (4038712) 4038712..4039263 - 552 WP_000102539.1 maltose O-acetyltransferase -
JSQ88_RS19175 (4039436) 4039436..4039654 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JSQ88_RS19180 (4039680) 4039680..4040054 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSQ88_RS19185 (4040599) 4040599..4043748 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
JSQ88_RS19190 (4043771) 4043771..4044964 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191300 WP_001291435.1 NZ_CP069621:c4039654-4039436 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191300 NZ_CP091673:3982008-3982472 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191300 WP_000344800.1 NZ_CP069621:c4040054-4039680 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191300 NZ_CP091673:3981673-3982008 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References