Detailed information of TA system TA191224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 986840..988560
• Replicon: chromosome
• Accession: NZ_CP069603
• Organism: Dickeya dianthicola strain 16LI01

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JRZ91_RS04610
• Protein ID: WP_103415840.1
• Coordinates: 987904..988560 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A3A4C958
• Locus tag: JRZ91_RS04605
• Protein ID: WP_024104822.1
• Coordinates: 986840..987907 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRZ91_RS04580 (JRZ91_04580)	981980..982306	-	327	Protein_894	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
JRZ91_RS04590 (JRZ91_04590)	982534..982716	-	183	WP_024109619.1	hypothetical protein	-
JRZ91_RS04595 (JRZ91_04595)	982865..983581	-	717	WP_103415838.1	SprT family zinc-dependent metalloprotease	-
JRZ91_RS04600 (JRZ91_04600)	983581..986676	-	3096	WP_103415839.1	HsdR family type I site-specific deoxyribonuclease	-
JRZ91_RS04605 (JRZ91_04605)	986840..987907	-	1068	WP_024104822.1	macro domain-containing protein	Antitoxin
JRZ91_RS04610 (JRZ91_04610)	987904..988560	-	657	WP_103415840.1	DUF4433 domain-containing protein	Toxin
JRZ91_RS04615 (JRZ91_04615)	988563..989138	-	576	WP_024104824.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
JRZ91_RS04620 (JRZ91_04620)	989143..990504	-	1362	WP_024104825.1	restriction endonuclease subunit S	-
JRZ91_RS04625 (JRZ91_04625)	990504..992939	-	2436	WP_024108177.1	type I restriction-modification system subunit M	-
JRZ91_RS04630 (JRZ91_04630)	992979..993218	-	240	WP_024104827.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25135.60 Da
• Isoelectric Point: 7.2459

>T191224 WP_103415840.1 NZ_CP069603:c988560-987904 [Dickeya dianthicola]
VAYDYSANLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAANWINIGNPELINRRAGHPVPAGAGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSSLRHVAIQNVPFVFTDSHAYYNLANYYTDLNNLHRIDWPILQARDFRRDQDD
LAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T191224 NZ_CP091662:3593090-3593368 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40767.89 Da
• Isoelectric Point: 5.4873

>AT191224 WP_024104822.1 NZ_CP069603:c987907-986840 [Dickeya dianthicola]
MMTYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYALACKQKQVTTGKMFITETDELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWSDVQARIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHNYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNNEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGECQPTLDSVKEG
LHQWPAGERWASRKMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT191224 NZ_CP091662:3592827-3593087 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3A4C958