Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 473862..474604 Replicon chromosome
Accession NZ_CP069603
Organism Dickeya dianthicola strain 16LI01

Toxin (Protein)


Gene name ataT Uniprot ID E0SIC1
Locus tag JRZ91_RS02150 Protein ID WP_013316117.1
Coordinates 473862..474341 (-) Length 160 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A3A4C8A5
Locus tag JRZ91_RS02155 Protein ID WP_024104363.1
Coordinates 474332..474604 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRZ91_RS02125 (JRZ91_02115) 469082..469405 + 324 WP_024104357.1 DNA-binding transcriptional regulator -
JRZ91_RS02130 (JRZ91_02120) 469692..471419 - 1728 WP_103415798.1 retron Ec67 family RNA-directed DNA polymerase/endonuclease -
JRZ91_RS02135 (JRZ91_02125) 472540..472860 + 321 WP_024104360.1 type II toxin-antitoxin system RelE/ParE family toxin -
JRZ91_RS02140 (JRZ91_02130) 472850..473134 + 285 WP_024104361.1 helix-turn-helix transcriptional regulator -
JRZ91_RS22230 473141..473287 + 147 Protein_414 transcriptional regulator -
JRZ91_RS02145 (JRZ91_02135) 473432..473833 - 402 WP_024104362.1 hypothetical protein -
JRZ91_RS02150 (JRZ91_02140) 473862..474341 - 480 WP_013316117.1 GNAT family N-acetyltransferase Toxin
JRZ91_RS02155 (JRZ91_02145) 474332..474604 - 273 WP_024104363.1 DUF1778 domain-containing protein Antitoxin
JRZ91_RS02160 (JRZ91_02150) 475094..475517 + 424 Protein_418 Arm DNA-binding domain-containing protein -
JRZ91_RS02170 (JRZ91_02160) 476440..476646 - 207 Protein_420 DUF1778 domain-containing protein -
JRZ91_RS22235 476717..476785 + 69 Protein_421 integrase -
JRZ91_RS22240 476810..476902 + 93 Protein_422 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JRZ91_RS02175 (JRZ91_02165) 476944..478332 + 1389 WP_024104366.1 hypothetical protein -
JRZ91_RS02180 (JRZ91_02170) 478482..478820 - 339 WP_024104367.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 461490..480096 18606
- flank IS/Tn - - 475948..476262 314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(28-147)

Antitoxin

Vibrio phage ICP1, Orf50

(10-85)


Sequences

Toxin        


Download         Length: 160 a.a.        Molecular weight: 17564.34 Da        Isoelectric Point: 9.5367

>T191221 WP_013316117.1 NZ_CP069603:c474341-473862 [Dickeya dianthicola]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Download         Length: 480 bp

>T191221 NZ_CP091662:2602532-2602639 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10390.84 Da        Isoelectric Point: 4.6702

>AT191221 WP_024104363.1 NZ_CP069603:c474604-474332 [Dickeya dianthicola]
MRTETKETPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRMN
ALLNRKSPWE

Download         Length: 273 bp

>AT191221 NZ_CP091662:c2602485-2602419 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N0G158

Antitoxin

Source ID Structure
AlphaFold DB A0A3A4C8A5

References