Detailed information of TA system TA191203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 473862..474604
• Replicon: chromosome
• Accession: NZ_CP069602
• Organism: Dickeya dianthicola strain 16LI02

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: E0SIC1
• Locus tag: JRZ85_RS02150
• Protein ID: WP_013316117.1
• Coordinates: 473862..474341 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3A4C8A5
• Locus tag: JRZ85_RS02155
• Protein ID: WP_024104363.1
• Coordinates: 474332..474604 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRZ85_RS02125 (JRZ85_02115)	469082..469405	+	324	WP_024104357.1	DNA-binding transcriptional regulator	-
JRZ85_RS02130 (JRZ85_02120)	469692..471419	-	1728	WP_103415798.1	retron Ec67 family RNA-directed DNA polymerase/endonuclease	-
JRZ85_RS02135 (JRZ85_02125)	472540..472860	+	321	WP_024104360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JRZ85_RS02140 (JRZ85_02130)	472850..473134	+	285	WP_024104361.1	helix-turn-helix transcriptional regulator	-
JRZ85_RS22235	473141..473287	+	147	Protein_414	transcriptional regulator	-
JRZ85_RS02145 (JRZ85_02135)	473432..473833	-	402	WP_024104362.1	hypothetical protein	-
JRZ85_RS02150 (JRZ85_02140)	473862..474341	-	480	WP_013316117.1	GNAT family N-acetyltransferase	Toxin
JRZ85_RS02155 (JRZ85_02145)	474332..474604	-	273	WP_024104363.1	DUF1778 domain-containing protein	Antitoxin
JRZ85_RS02160 (JRZ85_02150)	475094..475517	+	424	Protein_418	Arm DNA-binding domain-containing protein	-
JRZ85_RS02170 (JRZ85_02160)	476440..476646	-	207	Protein_420	DUF1778 domain-containing protein	-
JRZ85_RS22240	476717..476785	+	69	Protein_421	integrase	-
JRZ85_RS22245	476810..476902	+	93	Protein_422	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JRZ85_RS02175 (JRZ85_02165)	476944..478332	+	1389	WP_024104366.1	hypothetical protein	-
JRZ85_RS02180 (JRZ85_02170)	478482..478820	-	339	WP_024104367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	461490..480096	18606
	-	flank	IS/Tn	-	-	475948..476262	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17564.34 Da
• Isoelectric Point: 9.5367

>T191203 WP_013316117.1 NZ_CP069602:c474341-473862 [Dickeya dianthicola]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T191203 NZ_CP091659:c4513609-4513433 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10390.84 Da
• Isoelectric Point: 4.6702

>AT191203 WP_024104363.1 NZ_CP069602:c474604-474332 [Dickeya dianthicola]
MRTETKETPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRMN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT191203 NZ_CP091659:c4513387-4512971 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3N0G158

Antitoxin

• AlphaFold DB: A0A3A4C8A5