Detailed information of TA system TA191193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1862755..1863308
• Replicon: chromosome
• Accession: NZ_CP069601
• Organism: Dickeya dianthicola strain 16LI04

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A385XW46
• Locus tag: JRZ89_RS08585
• Protein ID: WP_024108344.1
• Coordinates: 1862994..1863308 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A385XYG1
• Locus tag: JRZ89_RS08580
• Protein ID: WP_024108345.1
• Coordinates: 1862755..1862991 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRZ89_RS08545 (JRZ89_08540)	1857839..1858252	-	414	WP_024108566.1	tautomerase family protein	-
JRZ89_RS08550 (JRZ89_08545)	1858317..1858727	-	411	WP_024108567.1	hypothetical protein	-
JRZ89_RS08555 (JRZ89_08550)	1858809..1859702	-	894	WP_024108568.1	alpha/beta hydrolase	-
JRZ89_RS08560 (JRZ89_08555)	1859711..1860694	-	984	WP_024108569.1	MBL fold metallo-hydrolase	-
JRZ89_RS08565 (JRZ89_08560)	1860960..1861445	+	486	WP_024108570.1	helix-turn-helix domain-containing protein	-
JRZ89_RS08570 (JRZ89_08565)	1861541..1861675	+	135	Protein_1681	winged helix-turn-helix transcriptional regulator	-
JRZ89_RS08575 (JRZ89_08570)	1861697..1862428	+	732	Protein_1682	zincin-like metallopeptidase domain-containing protein	-
JRZ89_RS08580 (JRZ89_08575)	1862755..1862991	+	237	WP_024108345.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JRZ89_RS08585 (JRZ89_08580)	1862994..1863308	+	315	WP_024108344.1	CcdB family protein	Toxin
JRZ89_RS08590 (JRZ89_08585)	1863951..1864469	+	519	WP_024108343.1	Hcp family type VI secretion system effector	-
JRZ89_RS08595 (JRZ89_08590)	1864582..1866438	+	1857	WP_024108342.1	type VI secretion system tip protein VgrG	-
JRZ89_RS22295	1866464..1866748	+	285	Protein_1687	type VI secretion system PAAR protein	-
JRZ89_RS08605 (JRZ89_08600)	1866988..1867863	+	876	WP_024108340.1	DUF6402 family protein	-
JRZ89_RS08610 (JRZ89_08605)	1867860..1868291	+	432	WP_071599229.1	DUF6201 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp	1852588..1877464	24876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11487.43 Da
• Isoelectric Point: 7.9859

>T191193 WP_024108344.1 NZ_CP069601:1862994-1863308 [Dickeya dianthicola]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T191193 NZ_CP091659:2695619-2695906 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9050.09 Da
• Isoelectric Point: 6.7184

>AT191193 WP_024108345.1 NZ_CP069601:1862755-1862991 [Dickeya dianthicola]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRRHEAKKWQEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT191193 NZ_CP091659:2695893-2696195 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A385XW46

Antitoxin

• AlphaFold DB: A0A385XYG1