Detailed information of TA system TA191172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1319344..1320172
• Replicon: chromosome
• Accession: NZ_CP069600
• Organism: Dickeya dianthicola strain 16MA15T

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A3A4CQM7
• Locus tag: JRZ90_RS06135
• Protein ID: WP_024105035.1
• Coordinates: 1319344..1319880 (-)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A385XV02
• Locus tag: JRZ90_RS06140
• Protein ID: WP_026594683.1
• Coordinates: 1319861..1320172 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRZ90_RS06115 (JRZ90_06115)	1314690..1315658	+	969	WP_024105031.1	o-succinylbenzoate synthase	-
JRZ90_RS06120 (JRZ90_06120)	1315646..1317016	+	1371	WP_024108474.1	o-succinylbenzoate--CoA ligase	-
JRZ90_RS06125 (JRZ90_06125)	1317223..1317768	+	546	WP_024105033.1	YfaZ family outer membrane protein	-
JRZ90_RS06130 (JRZ90_06130)	1317991..1319184	+	1194	WP_024105034.1	nicotinamide mononucleotide deamidase-related protein YfaY	-
JRZ90_RS06135 (JRZ90_06135)	1319344..1319880	-	537	WP_024105035.1	GNAT family N-acetyltransferase	Toxin
JRZ90_RS06140 (JRZ90_06140)	1319861..1320172	-	312	WP_026594683.1	DUF1778 domain-containing protein	Antitoxin
JRZ90_RS06145 (JRZ90_06145)	1320471..1320740	-	270	Protein_1204	IS4 family transposase	-
JRZ90_RS06150 (JRZ90_06150)	1320843..1321703	-	861	WP_024105037.1	co-chaperone YbbN	-
JRZ90_RS06155 (JRZ90_06155)	1321766..1322542	-	777	WP_024105038.1	SDR family oxidoreductase	-
JRZ90_RS06160 (JRZ90_06160)	1322606..1323247	-	642	WP_024108475.1	multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1	-
JRZ90_RS06165 (JRZ90_06165)	1323215..1323901	+	687	WP_024107942.1	putative ABC transporter ATP-binding protein YbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-154)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-97)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19327.27 Da
• Isoelectric Point: 6.7055

>T191172 WP_024105035.1 NZ_CP069600:c1319880-1319344 [Dickeya dianthicola]
MVDTQSESNGVVVYAYQADTTYPGSKSFDCGNTVINSFVRSSLKKSVRDGNCAAKVLVKDETKELLGFCTFAAYSLEKSK
LAGVVSGSLPHELGVVRLIMLGVATKEQNKGYGQELLLEFFKQVKTIHESLPVKGVYLDADPAAIDFYIRLGFVQLNEPP
NAFGAVPMFLAIQHILAA

Nucleotide

• Length: 537 bp

>T191172 NZ_CP091652:3664295-3664693 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11420.03 Da
• Isoelectric Point: 10.5508

>AT191172 WP_026594683.1 NZ_CP069600:c1320172-1319861 [Dickeya dianthicola]
MSNVSVFPNDIRVTKNARVELKTSPDLKDVLREAATAAGLDLSAFILNAALERAESVLDNQRRRQLSAASWQQLNQLLSE
PAAPTLALQALMQRKKQDGRYSK

Nucleotide

• Length: 312 bp

>AT191172 NZ_CP091652:3663999-3664292 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A3A4CQM7

Antitoxin

• AlphaFold DB: A0A385XV02