Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1319348..1320176 Replicon chromosome
Accession NZ_CP069599
Organism Dickeya dianthicola strain 16MB01

Toxin (Protein)


Gene name itaT Uniprot ID A0A3A4CQM7
Locus tag JRZ87_RS06135 Protein ID WP_024105035.1
Coordinates 1319348..1319884 (-) Length 179 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A385XV02
Locus tag JRZ87_RS06140 Protein ID WP_026594683.1
Coordinates 1319865..1320176 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRZ87_RS06115 (JRZ87_06105) 1314694..1315662 + 969 WP_024105031.1 o-succinylbenzoate synthase -
JRZ87_RS06120 (JRZ87_06110) 1315650..1317020 + 1371 WP_024108474.1 o-succinylbenzoate--CoA ligase -
JRZ87_RS06125 (JRZ87_06115) 1317227..1317772 + 546 WP_024105033.1 YfaZ family outer membrane protein -
JRZ87_RS06130 (JRZ87_06120) 1317995..1319188 + 1194 WP_024105034.1 nicotinamide mononucleotide deamidase-related protein YfaY -
JRZ87_RS06135 (JRZ87_06125) 1319348..1319884 - 537 WP_024105035.1 GNAT family N-acetyltransferase Toxin
JRZ87_RS06140 (JRZ87_06130) 1319865..1320176 - 312 WP_026594683.1 DUF1778 domain-containing protein Antitoxin
JRZ87_RS06145 (JRZ87_06135) 1320475..1320744 - 270 Protein_1204 IS4 family transposase -
JRZ87_RS06150 (JRZ87_06140) 1320847..1321707 - 861 WP_024105037.1 co-chaperone YbbN -
JRZ87_RS06155 (JRZ87_06145) 1321770..1322546 - 777 WP_024105038.1 SDR family oxidoreductase -
JRZ87_RS06160 (JRZ87_06150) 1322610..1323251 - 642 WP_024108475.1 multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1 -
JRZ87_RS06165 (JRZ87_06155) 1323219..1323905 + 687 WP_024107942.1 putative ABC transporter ATP-binding protein YbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-154)

Antitoxin

Vibrio phage ICP1, Orf50

(13-97)


Sequences

Toxin        


Download         Length: 179 a.a.        Molecular weight: 19327.27 Da        Isoelectric Point: 6.7055

>T191154 WP_024105035.1 NZ_CP069599:c1319884-1319348 [Dickeya dianthicola]
MVDTQSESNGVVVYAYQADTTYPGSKSFDCGNTVINSFVRSSLKKSVRDGNCAAKVLVKDETKELLGFCTFAAYSLEKSK
LAGVVSGSLPHELGVVRLIMLGVATKEQNKGYGQELLLEFFKQVKTIHESLPVKGVYLDADPAAIDFYIRLGFVQLNEPP
NAFGAVPMFLAIQHILAA

Download         Length: 537 bp

>T191154 NZ_CP091652:147251-147538 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11420.03 Da        Isoelectric Point: 10.5508

>AT191154 WP_026594683.1 NZ_CP069599:c1320176-1319865 [Dickeya dianthicola]
MSNVSVFPNDIRVTKNARVELKTSPDLKDVLREAATAAGLDLSAFILNAALERAESVLDNQRRRQLSAASWQQLNQLLSE
PAAPTLALQALMQRKKQDGRYSK

Download         Length: 312 bp

>AT191154 NZ_CP091652:147012-147251 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A4CQM7

Antitoxin

Source ID Structure
AlphaFold DB A0A385XV02

References