191120

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DhiAT/dnstrm_HI1420(antitoxin)
Location 1628439..1629192 Replicon chromosome
Accession NZ_CP069597
Organism Dickeya dianthicola strain 16ME22T

Toxin (Protein)


Gene name dhiT Uniprot ID A0A3A4D4W2
Locus tag JRZ88_RS07580 Protein ID WP_024105250.1
Coordinates 1628439..1628696 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID A0A3A4CVJ0
Locus tag JRZ88_RS07585 Protein ID WP_024108578.1
Coordinates 1628704..1629192 (+) Length 163 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JRZ88_RS07565 (JRZ88_07570) 1623690..1624898 - 1209 WP_026595017.1 ATP-binding protein -
JRZ88_RS07570 (JRZ88_07575) 1625216..1627366 - 2151 WP_024108577.1 formate dehydrogenase subunit alpha -
JRZ88_RS07575 (JRZ88_07580) 1627401..1627943 - 543 WP_013317225.1 4Fe-4S dicluster domain-containing protein -
JRZ88_RS07580 (JRZ88_07585) 1628439..1628696 + 258 WP_024105250.1 DUF4160 domain-containing protein Toxin
JRZ88_RS07585 (JRZ88_07590) 1628704..1629192 + 489 WP_024108578.1 addiction module antitoxin Antitoxin
JRZ88_RS07590 (JRZ88_07595) 1629327..1629824 - 498 WP_024108579.1 hydrogenase maturation peptidase HycI -
JRZ88_RS07595 (JRZ88_07600) 1629817..1630215 - 399 WP_024105253.1 formate hydrogenlyase maturation HycH family protein -
JRZ88_RS07600 (JRZ88_07605) 1630212..1631003 - 792 WP_024108580.1 NADH-quinone oxidoreductase subunit B family protein -
JRZ88_RS07605 (JRZ88_07610) 1631000..1631569 - 570 WP_103415738.1 hydrogenase 4 subunit H -
JRZ88_RS07610 (JRZ88_07615) 1631580..1633307 - 1728 WP_024105256.1 hydrogenase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Uncharacterized protein HI1420

(80-157)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10039.68 Da        Isoelectric Point: 8.9654

>T191120 WP_024105250.1 NZ_CP069597:1628439-1628696 [Dickeya dianthicola]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGHHELIISIRTGIEIEGYLPNNKRKLALVHIRAHRQQLLSMWERAVVGR
NPGKL

Download         Length: 258 bp

>T191120 NZ_CP091650:c2214731-2214447 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 163 a.a.        Molecular weight: 18053.72 Da        Isoelectric Point: 8.0587

>AT191120 WP_024108578.1 NZ_CP069597:1628704-1629192 [Dickeya dianthicola]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYHYAPSPVFSNASQFIKFGLLPDGSLEWLPDIKLSASELRKKGEYVSHTLA
VRERNFADVISRAFYDAVIENRPEILQAALKIAVEKYGNSTVAKKAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Download         Length: 489 bp

>AT191120 NZ_CP091650:c2214963-2214721 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A4D4W2

Antitoxin

Source ID Structure
AlphaFold DB A0A3A4CVJ0

References