Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65880..66405 Replicon plasmid unnamed1
Accession NZ_CP069590
Organism Escherichia coli strain FDAARGOS_1266

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag I6J96_RS25030 Protein ID WP_001159868.1
Coordinates 66100..66405 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6J96_RS25025 Protein ID WP_000813634.1
Coordinates 65880..66098 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J96_RS25000 61159..62772 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
I6J96_RS25005 62803..63153 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
I6J96_RS25010 63150..63575 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
I6J96_RS25015 63637..64788 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
I6J96_RS25020 64822..65334 - 513 WP_000151784.1 hypothetical protein -
I6J96_RS25025 65880..66098 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6J96_RS25030 66100..66405 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
I6J96_RS25035 66406..67212 + 807 WP_000016982.1 site-specific integrase -
I6J96_RS25040 67986..68741 + 756 WP_000852146.1 replication initiation protein RepE -
I6J96_RS25045 69329..70495 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / blaTEM-1B / tet(A) / blaCTX-M-15 - 1..127965 127965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T191069 WP_001159868.1 NZ_CP069590:66100-66405 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T191069 NZ_CP091632:5399-5914 [Salmonella enterica]
ATGAAATTTTACACTATATCAAGCAGATACATTGAATACCTAAAGGGATTCGAAGACAAAGTTCCCAATAGCGAAGATCC
TAACTATCAGAATCCGAAAGCTTTCATTGGCATTGTGTTAGAGATTCAAGGGCATAAGTATCTAGCCCCTTTAACATCCC
CAAAGCCATGGCATAGCACTATTAAAGAATCATCGCCTAGCTTCTTTAAGCTCCATGAAAATGGTGTCCCCGAAAATCAG
CTCGGATTGATTAATCTGAAGTTCATGATTCCAATCATCGAAGCCGAAGTATCCTTACTTGACTTGAACGGTATGCCGAA
TACCGCCTACAAGAGAATGCTCTATAAGCAGCTCCAGTTTATTCGTGTAAATTCAGACAAAATAGCTGAGAAATCAGAGC
TTTTAAGGAAGCTCGTCCTGCAAGGAAAAATGCAAGGAACATGTAATTTTTCCCTGTTAGAAGAAAAATACCGAGATTTC
GGCAAAGAAGCTAATGACACGGAAGAAGGCGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT191069 WP_000813634.1 NZ_CP069590:65880-66098 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT191069 NZ_CP091632:5257-5349 [Salmonella enterica]
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTAAGTGCAGCTAGAAATTTAGGTCATTT
ACTACCTTAAAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References