Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4685456..4686074 Replicon chromosome
Accession NZ_CP069589
Organism Escherichia coli strain FDAARGOS_1266

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J96_RS22785 Protein ID WP_001291435.1
Coordinates 4685456..4685674 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J96_RS22790 Protein ID WP_000344800.1
Coordinates 4685700..4686074 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J96_RS22750 4680743..4681315 + 573 WP_000779831.1 YbaY family lipoprotein -
I6J96_RS22755 4681346..4681657 - 312 WP_000409908.1 MGMT family protein -
I6J96_RS22765 4682036..4682389 + 354 WP_000878135.1 DUF1428 family protein -
I6J96_RS22770 4682431..4683981 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J96_RS22775 4684145..4684615 - 471 WP_000136192.1 YlaC family protein -
I6J96_RS22780 4684731..4685282 - 552 WP_000102539.1 maltose O-acetyltransferase -
I6J96_RS22785 4685456..4685674 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J96_RS22790 4685700..4686074 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J96_RS22795 4686620..4689769 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
I6J96_RS22800 4689792..4690985 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191064 WP_001291435.1 NZ_CP069589:c4685674-4685456 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191064 NZ_CP091630:3347770-3347988 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191064 WP_000344800.1 NZ_CP069589:c4686074-4685700 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191064 NZ_CP091630:3347368-3347742 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References