Detailed information of TA system TA191062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4234642..4235162
• Replicon: chromosome
• Accession: NZ_CP069589
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A061KX66
• Locus tag: I6J96_RS20645
• Protein ID: WP_001160964.1
• Coordinates: 4234878..4235162 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: I6J96_RS20640
• Protein ID: WP_000125566.1
• Coordinates: 4234642..4234875 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS20615	4229715..4230002	+	288	WP_000203744.1	ferredoxin-like protein FixX	-
I6J96_RS20620	4230061..4231392	+	1332	WP_001183209.1	MFS transporter	-
I6J96_RS20625	4231500..4232030	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
I6J96_RS20630	4232023..4233885	+	1863	WP_000377106.1	glutathione-regulated potassium-efflux system protein KefC	-
I6J96_RS20635	4234077..4234556	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
I6J96_RS20640	4234642..4234875	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I6J96_RS20645	4234878..4235162	+	285	WP_001160964.1	CcdB family protein	Toxin
I6J96_RS20650	4235189..4236037	-	849	WP_000257181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
I6J96_RS20655	4236044..4236421	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
I6J96_RS20660	4236424..4237245	-	822	WP_001065365.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
I6J96_RS20665	4237242..4238231	-	990	WP_000241227.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
I6J96_RS20670	4238231..4239517	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-93)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10692.54 Da
• Isoelectric Point: 10.3030

>T191062 WP_001160964.1 NZ_CP069589:4234878-4235162 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Nucleotide

• Length: 285 bp

>T191062 NZ_CP091630:c1014621-1014094 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGGGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT191062 WP_000125566.1 NZ_CP069589:4234642-4234875 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT191062 NZ_CP091630:c1014908-1014618 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A061KX66

Antitoxin

• AlphaFold DB: A0A7U9LTM9