Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4234642..4235162 Replicon chromosome
Accession NZ_CP069589
Organism Escherichia coli strain FDAARGOS_1266

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag I6J96_RS20645 Protein ID WP_001160964.1
Coordinates 4234878..4235162 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6J96_RS20640 Protein ID WP_000125566.1
Coordinates 4234642..4234875 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J96_RS20615 4229715..4230002 + 288 WP_000203744.1 ferredoxin-like protein FixX -
I6J96_RS20620 4230061..4231392 + 1332 WP_001183209.1 MFS transporter -
I6J96_RS20625 4231500..4232030 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J96_RS20630 4232023..4233885 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
I6J96_RS20635 4234077..4234556 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J96_RS20640 4234642..4234875 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J96_RS20645 4234878..4235162 + 285 WP_001160964.1 CcdB family protein Toxin
I6J96_RS20650 4235189..4236037 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J96_RS20655 4236044..4236421 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J96_RS20660 4236424..4237245 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J96_RS20665 4237242..4238231 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J96_RS20670 4238231..4239517 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T191062 WP_001160964.1 NZ_CP069589:4234878-4235162 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T191062 NZ_CP091630:c1014621-1014094 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGGGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT191062 WP_000125566.1 NZ_CP069589:4234642-4234875 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT191062 NZ_CP091630:c1014908-1014618 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References