Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3027250..3028050 Replicon chromosome
Accession NZ_CP069589
Organism Escherichia coli strain FDAARGOS_1266

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag I6J96_RS15015 Protein ID WP_000342452.1
Coordinates 3027250..3027777 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag I6J96_RS15020 Protein ID WP_001277107.1
Coordinates 3027784..3028050 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J96_RS14990 3022326..3023093 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
I6J96_RS14995 3023090..3024367 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
I6J96_RS15000 3024364..3025290 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
I6J96_RS15005 3025338..3026447 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
I6J96_RS15010 3026870..3027253 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
I6J96_RS15015 3027250..3027777 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
I6J96_RS15020 3027784..3028050 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
I6J96_RS15025 3028199..3029302 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
I6J96_RS15030 3029573..3030427 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
I6J96_RS15035 3030672..3031730 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
I6J96_RS15040 3031723..3032391 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T191055 WP_000342452.1 NZ_CP069589:c3027777-3027250 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T191055 NZ_CP091626:c4004513-4004229 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGACGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT191055 WP_001277107.1 NZ_CP069589:c3028050-3027784 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT191055 NZ_CP091626:c4004745-4004503 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References