191018

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4253233..4253809 Replicon chromosome
Accession NZ_CP069583
Organism Escherichia coli strain FDAARGOS_1265

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag I6J95_RS20940 Protein ID WP_001295743.1
Coordinates 4253522..4253809 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag I6J95_RS20935 Protein ID WP_000063148.1
Coordinates 4253233..4253535 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J95_RS20920 4249829..4251979 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
I6J95_RS20925 4252029..4252232 + 204 WP_000467859.1 YbdD/YjiX family protein -
I6J95_RS20930 4252243..4253199 + 957 WP_001295745.1 GTPase -
I6J95_RS20935 4253233..4253535 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
I6J95_RS20940 4253522..4253809 - 288 WP_001295743.1 BrnT family toxin Toxin
I6J95_RS20945 4254006..4255172 + 1167 WP_000800831.1 restriction endonuclease -
I6J95_RS20950 4255236..4256855 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
I6J95_RS20955 4256845..4258149 + 1305 WP_000535012.1 restriction endonuclease subunit S -
I6J95_RS20960 4258244..4258738 + 495 Protein_4111 type I restriction endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T191018 WP_001295743.1 NZ_CP069583:c4253809-4253522 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T191018 NZ_CP091619:317650-318003 [Salmonella enterica]
ATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGGCATGCCTGATCCTGG
GCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGTGGCTGCTGGCGGCAA
TGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTTATTACGCTGCTGTTT
TTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGCGGCAGGGCGGCTTAC
GCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT191018 WP_000063148.1 NZ_CP069583:c4253535-4253233 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT191018 NZ_CP091619:317416-317637 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References