Detailed information of TA system TA190999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 188556..189356
• Replicon: chromosome
• Accession: NZ_CP069583
• Organism: Escherichia coli strain FDAARGOS_1265

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: I6J95_RS00835
• Protein ID: WP_000342452.1
• Coordinates: 188829..189356 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: I6J95_RS00830
• Protein ID: WP_001277107.1
• Coordinates: 188556..188822 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J95_RS00810	184215..184883	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
I6J95_RS00815	184876..185934	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
I6J95_RS00820	186179..187033	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
I6J95_RS00825	187304..188407	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
I6J95_RS00830	188556..188822	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
I6J95_RS00835	188829..189356	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
I6J95_RS00840	189353..189736	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
I6J95_RS00845	190159..191268	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
I6J95_RS00850	191316..192242	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6J95_RS00855	192239..193516	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
I6J95_RS00860	193513..194280	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T190999 WP_000342452.1 NZ_CP069583:188829-189356 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T190999 NZ_CP091611:c1009338-1008811 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGGGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT190999 WP_001277107.1 NZ_CP069583:188556-188822 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT190999 NZ_CP091611:c1009625-1009335 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57